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Error when tissueproteomics tool is used

When I use the tissueproteomics tool, I get an error without any specification. All the data used in this command are open and in the same folder. I hoped that someone might see what causes the error…

root@739b55854fdf:/data# OpenSwathWorkflow -in 20190308_T1.mzML \

-tr 20190926_spectrallib_consensus_optimal_openswath.pqp
-tr_irt iRTassays.TraML
-swath_windows_file SWATHwindows_file_64_example_header_no-overlap.tsv
-batchSize 1000
-mz_extraction_window 50 -ppm
-rt_extraction_window 600
-Scoring:stop_report_after_feature 5
-readOptions cacheWorkingInMemory -tempDirectory /tmp/
-use_ms1_traces
-mz_correction_function quadratic_regression_delta_ppm
-RTNormalization:alignmentMethod lowess
-out_osw 20201211_NS_processed_T1.osw
-threads 20
Validate provided Swath windows file:
Read Swath window header start end
Read Swath window file with 64 SWATH windows.
Read Swath maps file with 64 windows.
Progress of ‘Load PQP file’:
– done [took 0.00 s (CPU), 0.01 s (Wall)] –
Loaded 0 proteins, 0 compounds with 0 transitions.
Loading mzML file 20190308_T1.mzML using readoptions cache
Progress of ‘Loading metadata file 20190308_T1.mzML’:
Will analyze the metadata first to determine the number of SWATH windows and the window sizes.
Determined there to be 64 SWATH windows and in total 2292 MS1 spectra
Determined there to be 64 SWATH windows and in total 2292 MS1 spectra
– done [took 01:42 m (CPU), 03:53 m (Wall)] –
Progress of ‘Loading data file 20190308_T1.mzML’:
Read chromatogram while reading SWATH files, did not expect that!

– done [took 34:52 m (CPU), 30:03 m (Wall)] –
Read Swath window header start end
Read Swath window file with 64 SWATH windows.
Re-annotate from file: SWATH 399.5 / 415.7 is annotated with 399.5 / 415.2
Re-annotate from file: SWATH 414.7 / 430.5 is annotated with 415.2 / 430

Re-annotate from file: SWATH 1074.3 / 1125.7 is annotated with 1074.8 / 1125.2
Re-annotate from file: SWATH 1124.7 / 1199.7 is annotated with 1125.2 / 1199.7
Will load iRT transitions and try to find iRT peptides

Progress of ‘Load TraML file’:

– done [took 0.23 s (CPU), 0.47 s (Wall)] –

Progress of ‘Extract iRT chromatograms’:

– done [took 36.04 s (CPU), 06:37 m (Wall)] –

Progress of ‘Retention time normalization’:
Will analyse 20 peptides with a total of 100 transitions
WARNING in SignalToNoiseEstimatorMedian: 1.48342% of all Signal-to-Noise estimates are too high, because the median was found in the rightmost histogram-bin. You should consider increasing ‘max_intensity’ (and maybe ‘bin_count’ with it, to keep bin width reasonable)
rsq: 0.422713 points: 20
rsq: 0.989652 points: 19

mz regression parameters: Y = 1.2606 + -0.00319267 X + 1.9698e-06 X^2

– done [took 1.61 s (CPU), 2.09 s (Wall)] –
Error: Unexpected internal error (file is encrypted or is not a database)